Harlington Manor is a house dating from the 16th century, though there is a possibility that it dates from the late 1390s and stands on a significantly earlier site. Major alterations were made in the 17th century, including panelling in the two principal reception rooms. In 1660, John Bunyan was interrogated by Sir Francis Wingate and briefly imprisoned in the house. Shortly afterwards, Charles II visited and stayed at the house. The Wingate family remained as the owners of the property until the mid 19th-century.

The house possesses a number of interesting architectural features. It has a very early 16th century wooden staircase leading to the attic. This is most unusual as most such staircases have been removed long ago. The attic itself is said to have two priest holes. The principal reception room (the great parlour) has a Tudor rose beam boss, Tudor beams and a Tudor fireplace. There is a very early 18th century staircase and a fine late Restoration dining room.

Reputedly, an exact replica of the house was constructed in Virginia, USA. Unfortunately, that house burned down in the late 19th century. There is a photograph of this house – but it does not look very similar to Harlington Manor. Certainly, however, the house was owned by the Burwell family, whose descendants became the biggest landowners in Virginia, governors of Virginia and even married into the Presidency.